Loughborough University
Leicestershire, UK
LE11 3TU
+44 (0)1509 222222
Loughborough University

Centre for Innovative and Collaborative Construction Engineering

2014


Dr Claire Camp

Thesis

Combining elemental and molecular mass spectrometry to study 3 types of biologically important compounds: DNA, phosphopeptides and anticancer drugs

Project Title

Improving the Detection Limits, Quantitation and Specificity of Mass Spectrometric Techniques for the Determination of Phosphorylated and Sulphur Containing Bio-molecules

Company

LGC Limited

Supervisors

Academic:
Professor Barry Sharp
Dr Helen Reid

Industrial:
Dr Heidi Goenaga-Infante
Dr Ruth Hearn

Director of Research:
Professor Stephen Ison

Research Period

2007 -2011

 

Improving the Detection Limits, Quantitation and Specificity of Mass Spectrometric Techniques for the Determination of Phosphorylated and Sulphur Containing Bio-molecules

Phosphorylated bio-molecules such as nucleic acids and post-translationally modified proteins represent a major group of analytes that are regularly studied in bio-medical research and drug development. Similarly there is interest in sulphur containing bio-molecules, notably proteins (S-containing amino acids are about 4% abundant in proteins), sulfurated proteins and sulphur containing drugs. Conventionally these are determined by LC and LC-MS, sometimes following separation by PAGE or immuno-affinity techniques.

ICP-MS offers an alternative for the assay of such compounds with significant advantages in relation to sensitivity and quantitation. Direct determination of the element provides the security of mass balance for both total and speciation measurements. Calibration is straightforward and can often be achieved using simple inorganic standards. Isotope dilution is readily implemented in both species specific and non-species specific mode. Additionally “tagging” techniques using hetero atoms or even nano-particles can be employed to greatly improve the DL and to introduce group selectivity into the ICP analysis without the use of high resolution separation techniques.

This project is specifically concerned with the development of new assays for P and S containing bio-molecules employing both collision-reaction cell ICP-MS and high-resolution ICP-MS combined with hetero atom tagging and novel calibration strategies.

Eslam M. Moustafa, Claire L. Camp, Ahmed S. Youssef, Asma Amleh, Helen J. Reid, Barry L. Sharp and Tamer Shoeib, Oxaliplatin Complexes with Carnosine and its Derivatives: In Vitro cytotoxicity, Mass Spectrometric and Computational Studies with a focus on Complex Fragmentation. Department of Chemistry, The American University in Cairo, New Cairo 11835, Egypt

C.L.Camp, B.l.Sharp, H.J. Reid, H. Goenga-Infante, J. Entwisle., 2011. Analysis of mono-phosphate nucleotides as a potential method for the quantification of DNA using high performance liquid chromatography-inductively coupled plasma-mass spectrometry. June 2011, Analytical and Bio-analytical Chemistry.

Biennial National Atomic Spectroscopy Symposium, (2008), Brighton.

European Winter Conference on Plasma Spectrochemistry (2009), Graz

 

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+44 (0)1509 222623

The Centre Administrator
CICE
Loughborough University
Leicestershire
LE11 3TU

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