Loughborough University
Leicestershire, UK
LE11 3TU
+44 (0)1509 263171
Loughborough University

Centre for Biological Engineering

Developing Scalable and Standardised Biomanufacturing Methods for Human Embryonic Stem Cells (hESCS)

CBE staff in laboratory

The work will deliver characterised, controlled and reproducible manufacturing-scale cell culture processes for the standardised culture and harvest of multiple pluripotent human embryonic stem cell (hESC) and induced pluripotency stem cell (iPSC) lines.

These processes are the critical platform technologies required for the banking of human pluripotent cells that is an absolute prerequisite for their use as products that are the technological endpoint for:

To scope maximal routes for application-specific pluripotent cell manufacture, we will compare both a completely automated, robotic system for T-flask-based culture and micro-carrier based culture in spinner flasks and STRs. Our multidisciplinary approach will improve the understanding, reproducibility and reliability of these, enabling robust and reproducible manufacture of pluripotent cell-based products at commercial scales.

Funding bodies

Project dates / duration

January 2009 - August 2012

Project team

Collaborators

Contact

Professor Christopher J Hewitt: C.J Hewitt@lboro.ac.uk, 01509 222506

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State-of-the-art facility

The facility is made up of a suite of Class 2 laboratories for cell growth, a bioelectrical facility and an analytical suite to service all laboratories.

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