Chemistry

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Nuclear Magnetic Resonance

NMR (Nuclear Magnetic Resonance) is a prominent technique for determining the structure of organic compounds. It is a non-destructive technique, which in most cases, requires samples of less than one milligram.

NMR can be used to study the structure of molecules, the interaction of various molecules, kinetics or dynamics of molecules, and the composition of mixtures of biological or synthetic solutions or composites.

The size of molecules of interest can range from a small organic molecule or metabolite, to a mid-sized peptide or natural product, all the way up to proteins of several tens of kDa in molecular weight.

The solid-state instrument can be booked on a sample by sample basis or for several days at a time. Prices are negotiable.

The instrument can also cope with gels, emulsions and suspensions using sealed glass inserts. Carbon-13 cross-polarisation magic-angle-spinning (CP-MAS) is the most commonly requested experiment and this can be achieved within an hour for favourable samples, however, highly crystalline samples can require up to 24h per sample.

Nuclear Magnetic Resonance Instruments available

Bruker DPX 400 spectrometer

The DPX 400 MHz spectrometer runs 24-7 with the aid of an auto-sampler and ICON-NMR, using XWIN-NMR 3.5 installed on a new PC. This is our open-access spectrometer and runs on a daily first-come first-served basis for proton during the day with 13C/DEPT running during the night. We have a 5mm normal geometry dual(1H 13C) gradients probe and a 5mm BBO probe. Standard experiments that are available include Proton, Carbon, DEPT, gCOSY, gTOCSY, gHMQC, gNOESY, variable temperature, homo-nuclear decoupling and nOe experiments.

Bruker Avance Ultra-Shield 400 spectrometer

The Avance US 400 MHz spectrometer runs 24-7 with the aid of an auto-sampler and ICON-NMR, using TOPSPIN installed on a new PC. This is a restricted access spectrometer. We have a 5mm auto-tune and matching BBO gradients probe. Standard experiments that are available include Proton, Carbon, DEPT, gCOSY, gTOCSY, gHMQC, gNOESY, variable temperature, homo-nuclear decoupling, gradients-nOe, shaped-pulse experiments, and diffusion experiments.

Bruker Avance 500, Solid-State NMR spectrometer

The solid-state 500 spectrometer is equipped with 100W and 500W amplifiers for the proton and X channel, and controlled by TOPSPIN installed on a new PC. We have a Bruker 2.5mm BBO MAS probe, a Bruker 4mm BBO MAS probe and a 5mm high-resolution BBO probe.